Evaluation of a modified Rose Bengal test and an indirect Enzyme-Linked Immunosorbent Assay for the diagnosis of Brucella melitensis infection in sheepReportar como inadecuado




Evaluation of a modified Rose Bengal test and an indirect Enzyme-Linked Immunosorbent Assay for the diagnosis of Brucella melitensis infection in sheep - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.



Abstract : A modified Rose Bengal test mRB and an indirect ELISA iELISA with Protein G as the conjugate, were evaluated for the diagnosis of Brucella melitensis infection in unvaccinated sheep with a known bacteriological status, and their diagnostic efficacy was compared with that of the standard Rose Bengal RB and Complement Fixation CF tests used in the current eradication campaign in EU countries. All tests showed 100% specificity when testing the sera from 212 Brucella-free sheep. When testing the sera from 219 Brucella melitensis culture-positive sheep, both the mRB and iELISA tests were more sensitive 98.6% and 96.8%, respectively than the RB and CF tests 95.0% and 92.7%, respectively. These results were similar when testing the sera from 181 animals belonging to infected flocks but found bacteriologically negative, suggesting that the mRB or iELISA tests could advantageously replace the current RB procedure used as the screening test.

Keywords : sheep Brucella melitensis serological diagnosis modified rose bengal test indirect ELISA





Autor: Ana Ferreira Regina Cardoso Isabel Dias Isabel Mariano Ana Belo Isabel Preto António Manteigas António Fonseca Maria Inácia Co

Fuente: https://hal.archives-ouvertes.fr/



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