Heterogeneity of DNA Distribution Pattern in Renal TumoursReport as inadecuate

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Analytical Cellular Pathology - Volume 14 1997, Issue 1, Pages 9-17

Institute of Pathology, University of Leipzig, Germany

Department of Urology, University of Leipzig, Germany

Received 17 September 1996; Revised 12 February 1997; Accepted 19 March 1997

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The presence of intratumoural heterogeneity in DNA distribution patterns has been accepted. However, most previous studies have not taken this fact into consideration. The value of DNA cytometry depends on its reproducibility. This could be influenced by heterogeneity failure. The aim of the present study is to evaluate intratumoural heterogeneity in renal cell cancer.

A sample of 22 tumours of the kidney was investigated by means of static DNA cytometry: 21 tumours were carcinomas, one was an angiomyolipoma. Probes from seven different locations of each tumour were Feulgen‐stained and measured. The variability of DNA features was determined and correlated with histological grade and type and with tumour size.

There was considerable intratumoural heterogeneity with respect to DNA distribution pattern in 45% of the tumours. Additional non‐diploid tumour‐stemlines and deviation of computed DNA features could be found in several cases by measuring more than one slide per tumour. A correlation between tumour heterogeneity, grading or typing, and tumour size could not be found.

Because these DNA parameters could serve as the foundation of a risk‐adapted treatment, tumour heterogeneity could have clinical consequences. Based on the results of this study we suggest measuring at least three slides per tumour to avoid misinterpretation of DNA measurements in renal cell cancer.

Author: Hans Nenning, Jörg Raßler, and Do Hoan Minh

Source: https://www.hindawi.com/


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