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Science China Life Sciences

, Volume 55, Issue 2, pp 158–163

First Online: 15 March 2012Received: 14 December 2011Accepted: 17 January 2012

Abstract

We developed a colorimetric assay to quantify clavulanic acid CA in culture broth of Streptomyces clavuligerus, to facilitate screening of a large number of S. clavuligerus mutants. The assay is based on a β-lactamase-catalyzed reaction, in which the yellow substrate nitrocefin λmax=390 nm is converted to a red product λmax=486 nm. Since CA can irreversibly inhibit β-lactamase activity, the level of CA in a sample can be measured as a function of the A390-A486 ratio in the assay mixture. The sensitivity and detection window of the assay were determined to be 50 μg L and 50 μg L to 10 mg L, respectively. The reliability of the assay was confirmed by comparing assay results with those obtained by HPLC. The assay was used to screen a pool of 65 S. clavuligerus mutants and was reliable for identifying CA over-producing mutants. Therefore, the assay saves time and labor in large-scale mutant screening and evaluation tasks. The detection window and the reliability of this assay are markedly better than those of previously reported CA assays. This assay method is suitable for high throughput screening of microbial samples and allows direct visual observation of CA levels on agar plates.

Keywordsclavulanic acid Streptomyces clavuligerus nitrocefin colorimetric assay β-lactamase Contributed equally to this work

This article is published with open access at Springerlink.com

Electronic Supplementary MaterialSupplementary material is available for this article at 10.1007-s11427-012-4287-x and is accessible for authorized users.

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Autor: XiDa Dai - SiHai Xiang - Jia Li - Qiang Gao - KeQian Yang

Fuente: https://link.springer.com/article/10.1007/s11427-012-4287-x



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