Xanthomonas oryzae pv oryzae triggers immediate transcriptomic modulations in riceReportar como inadecuado




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BMC Genomics

, 13:49

First Online: 31 January 2012Received: 08 October 2011Accepted: 31 January 2012

Abstract

BackgroundXanthomonas oryzae pv oryzae is a devastating pathogen of rice and has been extensively studied as a model pathogen of monocotyledons. Expressional studies in both the contenders have been undertaken in past to understand the molecular mechanism underlying the compatible and incompatible interactions in the pathosystem. Continuous update on database and gene annotations necessitates constant updating on the roles of the new entities as well as reinterpretation of regulations of the previous ones. Moreover the past endeavors have addressed the middle or late defense responses of the rice plant whereas in the present study an attempt has been made to investigate the early defense responses taking place immediately after inoculation.

ResultsMicroarray was used to study the transcriptional modulations in eighteen days old rice seedling leaves of both susceptible and resistant genotypes one hour after inoculation. In resistant plants as compared to susceptible ones 274 genes were found to be differentially expressed. Annotations could be assigned to 112 up- and 73 down-regulated transcripts and gene interaction maps were generated for 86 transcripts. Expressional data and interaction maps were used to develop a hypothetical scheme of the molecular events taking place during early defense response. Network analysis with the differential transcripts showed up-regulation of major clusters of cell signaling proteins and transcription factors while growth and basal metabolic components were largely found to be down-regulated.

ConclusionsThis study provides an understanding of the early defense signaling in rice cells. Components of the calcium and lipid signaling as well as MAPK cascade were modulated, by signals from surface receptors and cytosolic R-proteins, to arouse jasmonic acid and ethylene signaling and suppress auxin signaling through various transcription factors. Abscisic acid modulation was also evident through the expression regulation of transcription factors involved with its functions. Moreover adjustments in expression levels of components of primary as well as secondary metabolism, protein trafficking and turnout were apparent, highlighting the complexity of defense response.

List of abbreviationsABAAbsicisic Acid

AMTAmmonium transporter

APRActin related protein

ATLAlkyltransferase-like protein

AUX-IAAAuxin response-Indole-3-acetic acid induced proteins

CCD1Coiled coil DIX domain

DREB1BDrought responsive element binding protein 1B

HAK5High affinity K transporter 5

Hin1Harpin induced protein1

KUP3K uptake transporter 3

LRRLeucine rich repeats

LysMLysin motif

MAPKMitogen activated protein kinases

MAPKKKMAPK kinase kinase

MAX2More axillary branches 2 protein

NACNAM, ATAF1, 2, and CUC2 transcription factors family

NB-ARCNucleotide binding adaptor shared by APRF-1, R proteins and CED-4

PR-genesPathogenesis related genes

PTR2Peptide transpoter 2

RINGReally interesting new gene

SCFSkp, Cullin, F-box containing protein

SRP31Serine-argentine-rich protein 31

STP1Sugar transporter 1

Tic32Translocon of the inner chloroplast membrane 32.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-13-49 contains supplementary material, which is available to authorized users.

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Autor: Rumdeep K Grewal - Sumanti Gupta - Sampa Das

Fuente: https://link.springer.com/article/10.1186/1471-2164-13-49







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