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Chinese Science Bulletin

, Volume 56, Issue 24, pp 2523–2530

First Online: 06 August 2011Received: 02 March 2011Accepted: 20 June 2011

Abstract

Molecular studies using trace DNA, such as from museum specimens, ancient or forensic samples and samples obtained noninvasively, often have a common problem of low quality of DNA templates. Amplification errors, such as allelic dropout and false allele, may arise during polymerase chain reaction PCR using such samples. A mathematical model which treats homozygotes and heterozygotes discriminately has been developed to measure sample quality and compute the confidence level of using multiple-tube approaches. We use plucked hair samples collected from 26 individual Sichuan snub-nosed monkeys Rhinopithecus roxellana to test the model. In this case, a confidence level of 99% can be achieved by three positive PCRs. If the sample quality is very poor and requires many PCR replicates, an alternative multiple-step genotyping method is recommended. This model enables researchers to optimize experimental protocols through pilot studies and obtain reliable genetic information using noninvasive sampling method.

KeywordsSichuan snub-nosed monkey noninvasive sampling microsatellite genotyping errors polynomial distribution model These authors contributed equally to the work.

This article is published with open access at Springerlink.com

Electronic Supplementary MaterialSupplementary material is available for this article at 10.1007-s11434-011-4634-5 and is accessible for authorized users.

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Autor: Gang He - Kang Huang - SongTao Guo - WeiHong Ji - XiaoGuang Qi - Yi Ren - XueLin Jin - BaoGuo Li

Fuente: https://link.springer.com/article/10.1007/s11434-011-4634-5



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