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Biotechnology Research InternationalVolume 2011 2011, Article ID 454090, 8 pages

Research Article

Department of Botany, Patna University, Patna 800013, India

Department of Physics, A.N.College, Patna 800013, India

Thin Film Laboratory, Department of Physics, Indian Institute of Technology, New Delhi 110016, India

Department of Electrical and Electronic Engineering, SMIT, Sikkim, India

Department of Chemistry, Magadh Mahila College, Patna University, Patna 800013, India

Received 17 May 2010; Revised 14 July 2010; Accepted 19 August 2010

Academic Editor: Gabriel A. Monteiro

Copyright © 2011 Naheed Ahmad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A single-step environmental friendly approach is employed to synthesize silver nanoparticles. The biomolecules found in plants induce the reduction of Ag

ions from silver nitrate to silver nanoparticles AgNPs. UV-visible spectrum of the aqueous medium containing silver ions demonstrated a peak at 425 nm corresponding to the plasmon absorbance of silver nanoparticles. Transmission electron microscopy TEM showed the formation of well-dispersed silver nanoparticles in the range of 5–20 nm. X-ray diffraction XRD spectrum of the AgNPs exhibited 2θ values corresponding to the silver nanocrystal. The process of reduction is extracellular and fast which may lead to the development of easy biosynthesis of silver nanoparticles. Plants during glycolysis produce a large amount of H

ions along with NAD which acts as a strong redoxing agent; this seems to be responsible for the formation of AgNPs. Water-soluble antioxidative agents like ascorbic acids further seem to be responsible for the reduction of AgNPs. These AgNPs produced show good antimicrobial activity against common pathogens.

Autor: Naheed Ahmad, Seema Sharma, V. N. Singh, S. F. Shamsi, Anjum Fatma, and B. R. Mehta



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