microRNA-16 expression in HeLa cells is upregulated in cell-cycle and drug dependent mannerReportar como inadecuado




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Chinese Science Bulletin

, Volume 56, Issue 1, pp 34–38

First Online: 02 February 2011Received: 08 September 2010Accepted: 22 October 2010

Abstract

microRNAs are single-stranded, non-coding RNAs that regulate gene expression. The microRNA-16 family has been reported to be involved in cell-cycle regulation, which could also downregulate expression of multiple pro-proliferation genes. The present results demonstrated that miR-16 expression in HeLa cells increased when the cells were arrested during S-phase under methyl methanesulfate MMS treatment. This further resulted in downregulation of a target protein CDC25A, whereas miR-16 expression did not increase when HeLa cells were arrested during the MMS-treated G0-G1 or G2-M phase. Furthermore, when HeLa cells were arrested during S-phase with hydroxyurea treatment, miR-16 expression did not increase. These results suggest that expression levels of microRNAs in mammalian cells are delicately regulated under variable cellular conditions.

KeywordsmicroRNA cell cycle DNA damage methyl methanesulfate HeLa cells This article is published with open access at Springerlink.com

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Autor: Jie Li - JiaRui Wu

Fuente: https://link.springer.com/article/10.1007/s11434-010-4251-8







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