Stimulatory effect of Echinacea purpurea extract on the trafficking activity of mouse dendritic cells: revealed by genomic and proteomic analysesReportar como inadecuado




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BMC Genomics

, 11:612

First Online: 01 November 2010Received: 19 January 2010Accepted: 01 November 2010

Abstract

BackgroundSeveral Echinacea species have been used as nutraceuticals or botanical drugs for -immunostimulation-, but scientific evidence supporting their therapeutic use is still controversial. In this study, a phytocompound mixture extracted from the butanol fraction BF of a stem and leaf S+L extract of E. purpurea BF-S+L-Ep containing stringently defined bioactive phytocompounds was obtained using standardized and published procedures. The transcriptomic and proteomic effects of this phytoextract on mouse bone marrow-derived dendritic cells BMDCs were analyzed using primary cultures.

ResultsTreatment of BMDCs with BF-S+L-Ep did not significantly influence the phenotypic maturation activity of dendritic cells DCs. Affymetrix DNA microarray and bioinformatics analyses of genes differentially expressed in DCs treated with BF-S+L-Ep for 4 or 12 h revealed that the majority of responsive genes were related to cell adhesion or motility Cdh10, Itga6, Cdh1, Gja1 and Mmp8, or were chemokines Cxcl2, Cxcl7 or signaling molecules Nrxn1, Pkce and Acss1. TRANSPATH database analyses of gene expression and related signaling pathways in treated-DCs predicted the JNK, PP2C-α, AKT, ERK1-2 or MAPKAPK pathways as the putative targets of BF-S+L-Ep. In parallel, proteomic analysis showed that the expressions of metabolic-, cytoskeleton- or NF-κB signaling-related proteins were regulated by treatment with BF-S+L-Ep. In vitro flow cytometry analysis of chemotaxis-related receptors and in vivo cell trafficking assay further showed that DCs treated with BF-S+L-Ep were able to migrate more effectively to peripheral lymph node and spleen tissues than DCs treated as control groups.

ConclusionResults from this study suggest that BF-S+L-Ep modulates DC mobility and related cellular physiology in the mouse immune system. Moreover, the signaling networks and molecules highlighted here are potential targets for nutritional or clinical application of Echinacea or other candidate medicinal plants.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-11-612 contains supplementary material, which is available to authorized users.

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Autor: Shu-Yi Yin - Wen-Hsin Wang - Pei-Hsueh Wang - Kandan Aravindaram - Pei-Ing Hwang - Han-Ming Wu - Ning-Sun Yang

Fuente: https://link.springer.com/article/10.1186/1471-2164-11-612







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