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BMC Cell Biology

, 11:84

First Online: 28 October 2010Received: 08 June 2010Accepted: 28 October 2010

Abstract

BackgroundMcm10 protein is essential for initiation and elongation phases of replication. Human cells proteolyze Mcm10 during mitosis, presumably to ensure a single round of replication. It has been proposed that anaphase promoting complex ubiquitinates Mcm10 in late M and early G1 phases.

ResultsIn contrast to the previous work, we report that the degradation of Mcm10 is initiated at the onset of mitosis. Immunoblotting and immunofluorescence assays display that Mcm10 levels are low in all phases of mitosis. We report that Mcm10 degradation is not dependent on anaphase promoting complex. Further, the proteolysis in M-phase can be independently mediated by non-overlapping regions of Mcm10, apparently employing a redundant mechanism to ensure downregulation.

ConclusionsIt is believed that the proteolysis of Mcm10 during mitosis is a vital mechanism to prevent aberrant initiation of replication and the present study describes the regulation of Mcm10 during this phase of the cell-cycle.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2121-11-84 contains supplementary material, which is available to authorized users.

Manpreet Kaur, Aparna Sharma, Muntaz Khan contributed equally to this work.

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Autor: Manpreet Kaur - Aparna Sharma - Muntaz Khan - Ananya Kar - Sandeep Saxena

Fuente: https://link.springer.com/article/10.1186/1471-2121-11-84



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