Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver AbscessReportar como inadecuado




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BioMed Research International - Volume 2014 2014, Article ID 127453, 10 pages -

Research Article

Departamento de Medicina Experimental, Facultad de Medicina, UNAM, Dr. Balmis 148, Colonia Doctores, 06726 México, DF, Mexico

Departamento de Infectómica y Patogénesis Molecular, CINVESTAV, 07360 México, DF, Mexico

Departamento de Biología Celular, CINVESTAV, 07360 México, DF, Mexico

Received 4 December 2013; Revised 19 February 2014; Accepted 7 March 2014; Published 22 April 2014

Academic Editor: Marlene Benchimol

Copyright © 2014 Cecilia Ximénez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The role of calreticulin CRT in host-parasite interactions has recently become an important area of research. Information about the functions of calreticulin and its relevance to the physiology of Entamoeba parasites is limited. The present work demonstrates that CRT of both pathogenic E. histolytica and nonpathogenic E. dispar species specifically interacted with human C1q inhibiting the activation of the classical complement pathway. Using recombinant EhCRT protein, we demonstrate that CRT interaction site and human C1q is located at the N-terminal region of EhCRT. The immunofluorescence and confocal microscopy experiments show that CRT and human C1q colocalize in the cytoplasmic vesicles and near to the surface membrane of previously permeabilized trophozoites or are incubated with normal human serum which is known to destroy trophozoites. In the presence of peripheral mononuclear blood cells, the distribution of EhCRT and C1q is clearly over the surface membrane of trophozoites. Nevertheless, the level of expression of CRT in situ in lesions of amoebic liver abscess ALA in the hamster model is different in both Entamoeba species; this molecule is expressed in higher levels in E. histolytica than in E. dispar. This result suggests that EhCRT may modulate some functions during the early moments of the host-parasite relationship.





Autor: Cecilia Ximénez, Enrique González, Miriam E. Nieves, Angélica Silva-Olivares, Mineko Shibayama, Silvia Galindo-Gómez, Ja

Fuente: https://www.hindawi.com/



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