Jellyfish mucin may have potential disease-modifying effects on osteoarthritisReportar como inadecuado




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BMC Biotechnology

, 9:98

First Online: 08 December 2009Received: 07 April 2009Accepted: 08 December 2009

Abstract

BackgroundWe aimed to study the effects of intra-articular injection of jellyfish mucin qniumucin on articular cartilage degeneration in a model of osteoarthritis OA created in rabbit knees by resection of the anterior cruciate ligament. Qniumucin was extracted from Aurelia aurita moon jellyfish and Stomolophus nomurai Nomura-s jellyfish and purified by ion exchange chromatography. The OA model used 36 knees in 18 Japanese white rabbits. Purified qniumucin extracts from S. nomurai or A. aurita were used at 1 mg-ml. Rabbits were divided into four groups: a control C group injected with saline; a hyaluronic acid HA-only group H group; two qniumucin-only groups M groups; and two qniumucin + HA groups MH groups. One milligram of each solution was injected intra-articularly once a week for 5 consecutive weeks, starting from 4 weeks after surgery. Ten weeks after surgery, the articular cartilage was evaluated macroscopically and histologically.

ResultsIn the C and M groups, macroscopic cartilage defects extended to the subchondral bone medially and laterally. When the H and both MH groups were compared, only minor cartilage degeneration was observed in groups treated with qniumucin in contrast to the group without qniumucin. Histologically, densely safranin-O-stained cartilage layers were observed in the H and two MH groups, but cartilage was strongly maintained in both MH groups.

ConclusionAt the concentrations of qniumucin used in this study, injection together with HA inhibited articular cartilage degeneration in this model of OA.

List of abbreviationsOAosteoarthritis

HAhyaluronic acid

NSAIDsnonsteroidal anti-inflammatory drugs

BSEbovine spongiform encephalopathy

ACL-Tanterior cruciate ligament transaction

EDTAethylene diaminetetraacetic acid

SAFself-assembled film.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6750-9-98 contains supplementary material, which is available to authorized users.

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Autor: Naoshi Ohta - Masato Sato - Kiminori Ushida - Mami Kokubo - Takayuki Baba - Kayoko Taniguchi - Makoto Urai - Koji Kihira -

Fuente: https://link.springer.com/article/10.1186/1472-6750-9-98



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