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BMC Genetics

, 10:32

First Online: 05 July 2009Received: 09 October 2008Accepted: 05 July 2009

Abstract

BackgroundBoar taint is an unpleasant odour and flavour of the meat from some uncastrated male pigs primarily caused by elevated levels of androstenone and skatole in adipose tissue.
Androstenone is produced in the same biochemical pathway as testosterone and estrogens, which represents a particular challenge when selecting against high levels of androstenone in the breeding programme, without simultaneously decreasing levels of other steroids.
Detection of single nucleotide polymorphisms SNPs associated with compounds affecting boar taint is important both for gaining a better understanding of the complex regulation of the trait and for the purpose of identifying markers that can be used to improve the gain of breeding.
The beneficial SNPs to be used in breeding would have the combinational effects of reducing levels of boar taint without affecting fertility of the animals.
The aim of this study was to detect SNPs in boar taint candidate genes and to perform association studies for both single SNPs and haplotypes with levels of boar taint compounds and phenotypes related to reproduction.

ResultsAn association study involving 275 SNPs in 121 genes and compounds related to boar taint and reproduction were carried out in Duroc and Norwegian Landrace boars.
Phenotypes investigated were levels of androstenone, skatole and indole in adipose tissue, levels of androstenone, testosterone, estrone sulphate and 17β-estradiol in plasma, and length of bulbo urethralis gland.
The SNPs were genotyped in more than 2800 individuals and several SNPs were found to be significantly LRT > 5.4 associated with the different phenotypes.
Genes with significant SNPs in either of the traits investigated include cytochrome P450 members CYP2E1, CYP21, CYP2D6 and CYP2C49, steroid 5α-reductase SRD5A2, nuclear receptor NGFIB, catenin CTNND1, BRCA1 associated protein BAP1 and hyaluronoglucosaminidase HYAL2.
Haplotype analysis provided additional evidence for an effect of CYP2E1 on levels of skatole and indole, and for BAP1, HYAL2 and SRD5A2 on levels of androstenone.

ConclusionThe findings in this study indicate that polymorphisms in CYP2E1, CYP21, CYP2D6, CYP2C49, NGFIB and CTNND1 might be used to reduce levels of boar taint without affecting levels of testosterone, estrone sulphate, 17β-estradiol or length of bulbo urethralis gland.

Abbreviations3β-HSD3β-hydroxysteroid dehydrogenase

AKR1C3aldo-keto reductase family member 1C3

AKR1C4aldo-keto reductase family member 1C4

AOXaldehyde oxidase

BAP1BRCA1 associated protein

CTNND1catenin delta

CYB5cytochrome b5

CYP17cytochrome P450 family member 17

CYP21cytochrome P450 family member 21

CYP2C49cytochrome P450 family member 2C49

CYP2D6cytochrome P450 family member 2D6

CYP2E1cytochrome P450 family member 2E1

DHRS8short-chain dehydrogenase-reductase member 8

DDuroc

FDX1ferredoxin

FTH1ferritin heavy polypeptide

HYAL1hyaluronoglucosaminidase 1

HYAL2hyaluronoglucosaminidase 2

HYAL3hyaluronoglucosaminidase 3

LDlinkage disequilibrium

MAFminor allele frequency

NGFIBorphan nuclear receptor member NGFI-B

NLNorwegian Landrace

SNPsingle nucleotide polymorphism

SRD5A2steroid 5α-reductase

StARsteroidogenic acute regulatory protein

UCHubiquitin carboxy-terminal hydrolase.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2156-10-32 contains supplementary material, which is available to authorized users.

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Autor: Maren Moe - Sigbjørn Lien - Torunn Aasmundstad - Theo HE Meuwissen - Marianne HS Hansen - Christian Bendixen - Eli Grind

Fuente: https://link.springer.com/article/10.1186/1471-2156-10-32



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