DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markersReportar como inadecuado




DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markers - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Microbiology

, 9:92

First Online: 13 May 2009Received: 10 November 2008Accepted: 13 May 2009

Abstract

BackgroundThe lipopolysaccharide is a major antigen and virulence factor of Brucella, an important bacterial pathogen. In smooth brucellae, lipopolysaccharide is made of lipid A-core oligosaccharide and N-formylperosamine O-polysaccharide. B. ovis and B. canis rough species lack the O-polysaccharide.

ResultsThe polymorphism of O-polysaccharide genes wbkE, manAO-Ag, manBO-Ag, manCO-Ag, wbkF and wbkD and wbo wboA and wboB, and core genes manBcoreand wa** was analyzed. Although most genes were highly conserved, species- and biovar-specific restriction patterns were found. There were no significant differences in putative N-formylperosamyl transferase genes, suggesting that Brucella A and M serotypes are not related to specific genes. In B. pinnipedialis and B. ceti both smooth, manBO-Agcarried an IS711, confirming its dispensability for perosamine synthesis. Significant differences between smooth and rough species were found in wbkF and wbkD, two adjacent genes putatively related to bactoprenol priming for O-polysaccharide polymerization. B. ovis wbkF carried a frame-shift and B. canis had a long deletion partially encompassing both genes. In smooth brucellae, this region contains two direct repeats suggesting the deletion mechanism.

ConclusionThe results define species and biovar markers, confirm the dispensability of manBO-Agfor O-polysaccharide synthesis and contribute to explain the lipopolysaccharide structure of rough and smooth Brucella species.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-9-92 contains supplementary material, which is available to authorized users.

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Autor: Michel S Zygmunt - José M Blasco - Jean-Jacques Letesson - Axel Cloeckaert - Ignacio Moriyón

Fuente: https://link.springer.com/article/10.1186/1471-2180-9-92



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