Up-regulation of uPARAP-Endo180 during culture activation of rat hepatic stellate cells and its presence in hepatic stellate cell lines from different speciesReportar como inadecuado




Up-regulation of uPARAP-Endo180 during culture activation of rat hepatic stellate cells and its presence in hepatic stellate cell lines from different species - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Cell Biology

, 10:39

First Online: 11 May 2009Received: 18 November 2008Accepted: 11 May 2009

Abstract

BackgroundThe urokinase plasminogen activator receptor associated protein uPARAP-Endo180 is a novel endocytic receptor that mediates collagen uptake and is implicated to play a role in physiological and pathological tissue-remodelling processes by mediating intracellular collagen degradation.

ResultThis study investigates the expression of uPARAP-Endo180 protein and messenger RNA in primary rat hepatic stellate cell HSC cultures. The results show that uPARAP-Endo180 protein is not expressed in freshly isolated HSCs or during the first few days of culture while the cells still display quiescent features. In contrast, uPARAP-Endo180 protein is expressed early during HSC activation when cells are transdifferentiated into myofibroblast-like cells. Very low levels of uPARAP-Endo180 mRNA are detectable during the first days of culture but uPARAP-Endo180 mRNA is strongly up-regulated with increasing time in culture. Moreover, endocytic uptake of denatured collagen increases as transdifferentiation proceeds over time and correlates with increased expression of uPARAP-Endo180. Finally, analysis of uPARAP-Endo180 expression in four hepatic stellate cell lines from three different species showed that all these cell lines express uPARAP-Endo180 and are able to take up denatured collagen efficiently.

ConclusionThese results demonstrate that uPARAP-Endo180 expression by rat HSCs is strongly up-regulated during culture activation and identify this receptor as a feature common to culture-activated HSCs.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2121-10-39 contains supplementary material, which is available to authorized users.

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Autor: Seyed A Mousavi - Marita S Fønhus - Trond Berg

Fuente: https://link.springer.com/article/10.1186/1471-2121-10-39







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