Comparative genomics of Toll-like receptor signalling in five speciesReportar como inadecuado

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BMC Genomics

, 10:216

First Online: 11 May 2009Received: 07 November 2008Accepted: 11 May 2009


BackgroundOver the last decade, several studies have identified quantitative trait loci QTL affecting variation of immune related traits in mammals. Recent studies in humans and mice suggest that part of this variation may be caused by polymorphisms in genes involved in Toll-like receptor TLR signalling. In this project, we used a comparative approach to investigate the importance of TLR-related genes in comparison with other immunologically relevant genes for resistance traits in five species by associating their genomic location with previously published immune-related QTL regions.

ResultsWe report the genomic localisation of TLR1-10 and ten associated signalling molecules in sheep and pig using in-silico and-or radiation hybrid RH mapping techniques and compare their positions with their annotated homologues in the human, cattle and mouse whole genome sequences. We also report medium-density RH maps for porcine chromosomes 8 and 13. A comparative analysis of the positions of previously published relevant QTLs allowed the identification of homologous regions that are associated with similar health traits in several species and which contain TLR related and other immunologically relevant genes. Additional evidence was gathered by examining relevant gene expression and association studies.

ConclusionThis comparative genomic approach identified eight genes as potentially causative genes for variations of health related traits. These include susceptibility to clinical mastitis in dairy cattle, general disease resistance in sheep, cattle, humans and mice, and tolerance to protozoan infection in cattle and mice. Four TLR-related genes TLR1, 6, MyD88, IRF3 appear to be the most likely candidate genes underlying QTL regions which control the resistance to the same or similar pathogens in several species. Further studies are required to investigate the potential role of polymorphisms within these genes.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-10-216 contains supplementary material, which is available to authorized users.

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Autor: Oliver C Jann - Annemarie King - Nestor Lopez Corrales - Susan I Anderson - Kirsty Jensen - Tahar Ait-ali - Haizhou Tang


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