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Clinical and Developmental ImmunologyVolume 2012 2012, Article ID 934161, 14 pages

Research Article

Histocompatibility Centre of Coimbra, Edifício São Jerónimo, 4 Piso, Praceta Mota Pinto, 3001-301 Coimbra, Portugal

College of Health Technology of Coimbra, S. Martinho do Bispo, 3046-854 Coimbra, Portugal

Rheumatology Department, University Hospital of Coimbra, 3000-075 Coimbra, Portugal

Faculty of Health Sciences, University of Beira Interior, 6200-506 Covilhã, Portugal

Faculty of Medicine, University of Coimbra, 3004-504 Coimbra, Portugal

Received 10 May 2012; Revised 10 July 2012; Accepted 17 July 2012

Academic Editor: Timothy B. Niewold

Copyright © 2012 Tiago Carvalheiro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abnormalities in monocytes and in peripheral blood dendritic cells DC subsets have been reported in systemic lupus erythematosus SLE. We aim to clarify the tolerogenic or inflammatory role of these cells based on ICOSL or IFN-α and chemokine mRNA expression, respectively, after cell purification. The study included 18 SLE patients with active disease ASLE, 25 with inactive disease ISLE, and 30 healthy controls HG. In purified plasmacytoid DC pDC was observed a lower ICOSL mRNA expression in ASLE and an increase in ISLE; similarly, a lower ICOSL mRNA expression in monocytes of ALSE patients was found. However, a higher ICOSL mRNA expression was observed in ASLE compared to HG in myeloid DCs. Interestingly, clinical parameters seem to be related with ICOSL mRNA expression. Regarding the inflammatory activity it was observed in purified monocytes and CD 1 4 − - l o w CD16

DCs an increase of CCL2, CXCL9, and CXCL10 mRNA expression in ASLE compared to HG. In myeloid DC no differences were observed regarding chemokines, and IFN-α mRNA expression. In pDC, a higher IFN-α mRNA expression was observed in ASLE. Deviations in ICOSL, chemokine, and IFN-α mRNA expression in peripheral blood monocytes and dendritic cells subpopulations in SLE appear to be related to disease activity.

Autor: Tiago Carvalheiro, Ana Rodrigues, Ana Lopes, Luís Inês, Isabel Velada, Andreia Ribeiro, António Martinho, José A. P. Si



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