Endothelium-Dependent Vasorelaxant Effect of Butanolic Fraction from Caryocar brasiliense Camb. Leaves in Rat Thoracic AortaReportar como inadecuado




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Evidence-Based Complementary and Alternative MedicineVolume 2012 2012, Article ID 934142, 9 pages

Research Article

Department of Physiological Sciences, Laboratory of Molecular and Biochemistry Pharmacology, Institute of Biological Sciences, Federal University of Goiás, Campus Samambaia, Sala 215, 74001-970 Goiânia, GO, Brazil

Department of Physiological Sciences, Laboratory of Autonomic and Cardiac Physiology, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil

Department of Pharmacy, Study Center in Biopharmacy, Federal University of Paraná, 80210-170 Curitiba, PR, Brazil

Department of Physiological Sciences, Laboratory of Physiology of Isolated Organs, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil

Department of Pharmacology, Laboratory of Hypertension, University of São Paulo, 05508-900 São Paulo, SP, Brazil

Department of Physiological Sciences, Laboratory of Pharmacology of Natural Products, Federal University of Goiás, 74001-970 Goiânia, GO, Brazil

Received 13 April 2012; Revised 20 June 2012; Accepted 10 July 2012

Academic Editor: Jairo Kenupp Bastos

Copyright © 2012 Lais Moraes de Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Caryocar brasiliense Camb. “pequi” is a native plant from the Cerrado region of Brazil that contains bioactive components reported to be antioxidant agents. Previous work has demonstrated that dietary supplementation with pequi decreased the arterial pressure of volunteer athletes. We found that the crude hydroalcoholic extract CHE of C. brasiliense leaves relaxed, in a concentration-dependent manner, rat aortic rings precontracted with phenylephrine, and that the butanolic fraction BF produced an effect similar to that of the CHE. Aortic relaxation induced by BF was abolished by endothelium removal, by incubation of the nitric oxide synthase inhibitor L-NAME, or the soluble guanylatecyclase inhibitor ODQ. However, incubation with atropine and pyrilamine had no effect on the BF-induced vasorelaxation. Moreover, this effect was not inhibited by indomethacin and tetraethylammonium. The concentration-response curve to calcium in denuded-endothelium rings was not modified after incubation with BF, and the vasorelaxation by BF in endothelium-intact rings precontracted with KCl was abolished after incubation with L-NAME. In addition, administration of BF in anesthetized rats resulted in a reversible hypotension. The results reveal that C. brasiliense possesses both in vivo and in vitro activities and that the vascular effect of BF involves stimulation of the nitric oxide-cyclic GMP pathway.





Autor: Lais Moraes de Oliveira, Aline Gabriela Rodrigues, Elaine Fernanda da Silva, Letícia Bonancio Cerqueira, Carlos Henrique Cast

Fuente: https://www.hindawi.com/



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