Myosin-Vb functions as a dynamic tether for peripheral endocytic compartments during transferrin traffickingReportar como inadecuado

Myosin-Vb functions as a dynamic tether for peripheral endocytic compartments during transferrin trafficking - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Cell Biology

, 9:44

First Online: 07 August 2008Received: 19 February 2008Accepted: 07 August 2008


BackgroundMyosin-Vb has been shown to be involved in the recycling of diverse proteins in multiple cell types. Studies on transferrin trafficking in HeLa cells using a dominant-negative myosin-Vb tail fragment suggested that myosin-Vb was required for recycling from perinuclear compartments to the plasma membrane. However, chemical-genetic, dominant-negative experiments, in which myosin-Vb was specifically induced to bind to actin, suggested that the initial hypothesis was incorrect both in its site and mode of myosin-Vb action. Instead, the chemical-genetic data suggested that myosin-Vb functions in the actin-rich periphery as a dynamic tether on peripheral endosomes, retarding transferrin transport to perinuclear compartments.

ResultsIn this study, we employed both approaches, with the addition of overexpression of full-length wild-type myosin-Vb and switching the order of myosin-Vb inhibition and transferrin loading, to distinguish between these hypotheses. Overexpression of full-length myosin-Vb produced large peripheral endosomes. Chemical-genetic inhibition of myosin-Vb after loading with transferrin did not prevent movement of transferrin from perinuclear compartments; however, virtually all myosin-Vb-decorated particles, including those moving on microtubules, were halted by the inhibition. Overexpression of the myosin-Vb tail caused a less-peripheral distribution of early endosome antigen-1 EEA1.

ConclusionAll results favored the peripheral dynamic tethering hypothesis.

Abbreviations ListEEA1early endosomal antigen-1

and PE-ADPN -2-phenylethyl-ADP.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2121-9-44 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Autor: D William ProvanceJr - Erin J Addison - Patrick R Wood - David Z Chen - Colleen M Silan - John A Mercer


Documentos relacionados