Cold-inducible RNA binding protein CIRP, a novel XTcf-3 specific target gene regulates neural development in XenopusReportar como inadecuado

Cold-inducible RNA binding protein CIRP, a novel XTcf-3 specific target gene regulates neural development in Xenopus - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Developmental Biology

, 8:77

First Online: 07 August 2008Received: 09 April 2008Accepted: 07 August 2008


BackgroundAs nuclear mediators of wnt-β-catenin signaling, Lef-Tcf transcription factors play important roles in development and disease. Although it is well established, that the four vertebrate Lef-Tcfs have unique functional properties, most studies unite Lef-1, Tcf-1, Tcf-3 and Tcf-4 and reduce their function to uniformly transduce wnt-β-catenin signaling for activating wnt target genes. In order to discriminate target genes regulated by XTcf-3 from those regulated by XTcf-4 or Lef-Tcfs in general, we performed a subtractive screen, using neuralized Xenopus animal cap explants.

ResultsWe identified cold-inducible RNA binding protein CIRP as novel XTcf-3 specific target gene. Furthermore, we show that knockdown of XTcf-3 by injection of an antisense morpholino oligonucleotide results in a general broadening of the anterior neural tissue. Depletion of XCIRP by antisense morpholino oligonucleotide injection leads to a reduced stability of mRNA and an enlargement of the anterior neural plate similar to the depletion of XTcf-3.

ConclusionDistinct steps in neural development are differentially regulated by individual Lef-Tcfs. For proper development of the anterior brain XTcf-3 and the Tcf-subtype specific target XCIRP appear indispensable. Thus, regulation of anterior neural development, at least in part, depends on mRNA stabilization by the novel XTcf-3 target gene XCIRP.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-8-77 contains supplementary material, which is available to authorized users.

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Autor: Stephanie van Venrooy - Dagmar Fichtner - Martin Kunz - Doris Wedlich - Dietmar Gradl


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