Genomic and bioinformatics analysis of human adenovirus type 37: New insights into corneal tropismReportar como inadecuado

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BMC Genomics

, 9:213

First Online: 09 May 2008Received: 07 December 2007Accepted: 09 May 2008


BackgroundHuman adenovirus type 37 HAdV-37 is a major etiologic agent of epidemic keratoconjunctivitis, a common and severe eye infection associated with long-term visual morbidity due to persistent corneal inflammation. While HAdV-37 has been known for over 20 years as an important cause, the complete genome sequence of this serotype has yet to be reported. A detailed bioinformatics analysis of the genome sequence of HAdV-37 is extremely important to understanding its unique pathogenicity in the eye.

ResultsWe sequenced and annotated the complete genome of HAdV-37, and performed genomic and bioinformatics comparisons with other HAdVs to identify differences that might underlie the unique corneal tropism of HAdV-37. Global pairwise genome alignment with HAdV-9, a human species D adenovirus not associated with corneal infection, revealed areas of non-conserved sequence principally in genes for the virus fiber site of host cell binding, penton host cell internalization signal, hexon principal viral capsid structural protein, and E3 site of several genes that mediate evasion of the host immune system. Phylogenetic analysis revealed close similarities between predicted proteins from HAdV-37 of species D and HAdVs from species B and E. However, virtual 2D gel analyses of predicted viral proteins uncovered unexpected differences in pI and-or size of specific proteins thought to be highly similar by phylogenetics.

ConclusionThis genomic and bioinformatics analysis of the HAdV-37 genome provides a valuable tool for understanding the corneal tropism of this clinically important virus. Although disparities between HAdV-37 and other HAdV within species D in genes encoding structural and host receptor-binding proteins were to some extent expected, differences in the E3 region suggest as yet unknown roles for this area of the genome. The whole genome comparisons and virtual 2D gel analyses reported herein suggest potent areas for future studies.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-9-213 contains supplementary material, which is available to authorized users.

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Autor: Christopher M Robinson - Fatemeh Shariati - Allison F Gillaspy - David W Dyer - James Chodosh


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