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BMC Developmental Biology

, 8:51

First Online: 09 May 2008Received: 18 November 2007Accepted: 09 May 2008


BackgroundDss1 or Rpn15 is a recently identified subunit of the 26S proteasome regulatory particle. In addition to its function in the protein degradation machinery, it has been linked to BRCA2 breast cancer susceptibility gene 2 product and homologous DNA recombination, mRNA export, and exocytosis. While the fungal orthologues of Dss1 are not essential for viability, the significance of Dss1 in metazoans has remained unknown due to a lack of knockout animal models.

ResultsIn the current study deletion of dss-1 was studied in Caenorhabditis elegans with a dss-1 loss-of-function mutant and dss-1 directed RNAi. The analysis revealed an essential role for dss-1 in oogenesis. In addition, dss-1 RNAi caused embryonic lethality and larval arrest, presumably due to loss of the dss-1 mRNA maternal contribution. DSS-1::GFP fusion protein localised primarily in the nucleus. No apparent effect on proteasome function was found in dss-1 RNAi treated worms. However, expression of the C. elegans dss-1 in yeast cells deleted for its orthologue SEM1 rescued their temperature-sensitive growth phenotype, and partially rescued the accumulation of polyubiquitinated proteins in these cells.

ConclusionThe first knockout animal model for the gene encoding the proteasome subunit DSS-1-Rpn15-Sem1 is characterised in this study. In contrast to unicellular eukaryotes, the C. elegans dss-1 encodes an essential protein, which is required for embryogenesis, larval growth, and oogenesis, and which is functionally conserved with its yeast and human homologues.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-8-51 contains supplementary material, which is available to authorized users.

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Autor: Johanna Pispa - Susanna Palmén - Carina I Holmberg - Jussi Jäntti

Fuente: https://link.springer.com/article/10.1186/1471-213X-8-51

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