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BMC Genomics

, 9:58

First Online: 30 January 2008Received: 11 June 2007Accepted: 30 January 2008


BackgroundMusa species Zingiberaceae, Zingiberales including bananas and plantains are collectively the fourth most important crop in developing countries. Knowledge concerning Musa genome structure and the origin of distinct cultivars has greatly increased over the last few years. Until now, however, no large-scale analyses of Musa genomic sequence have been conducted. This study compares genomic sequence in two Musa species with orthologous regions in the rice genome.

ResultsWe produced 1.4 Mb of Musa sequence from 13 BAC clones, annotated and analyzed them along with 4 previously sequenced BACs. The 443 predicted genes revealed that Zingiberales genes share GC content and distribution characteristics with eudicot and Poaceae genomes. Comparison with rice revealed microsynteny regions that have persisted since the divergence of the Commelinid orders Poales and Zingiberales at least 117 Mya. The previously hypothesized large-scale duplication event in the common ancestor of major cereal lineages within the Poaceae was verified. The divergence time distributions for Musa-Zingiber Zingiberaceae, Zingiberales orthologs and paralogs provide strong evidence for a large-scale duplication event in the Musa lineage after its divergence from the Zingiberaceae approximately 61 Mya. Comparisons of genomic regions from M. acuminata and M. balbisiana revealed highly conserved genome structure, and indicated that these genomes diverged circa 4.6 Mya.

ConclusionThese results point to the utility of comparative analyses between distantly-related monocot species such as rice and Musa for improving our understanding of monocot genome evolution. Sequencing the genome of M. acuminata would provide a strong foundation for comparative genomics in the monocots. In addition a genome sequence would aid genomic and genetic analyses of cultivated Musa polyploid genotypes in research aimed at localizing and cloning genes controlling important agronomic traits for breeding purposes.

Abbreviationsaaamino acid

AC numberaccession number

CDScoding DNA sequence

ESTexpressed sequence tag

FISHfluorescent in situ hybridization

GISHgenomic in situ hybridization

Kssynonymous substitution rate

Myamillion years ago

PKWPisang Klutuk Wulung

RFLPrestriction fragment length polymorphism

TEtransposable element

TCtentative consensi.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-9-58 contains supplementary material, which is available to authorized users.

Magali Lescot, Pietro Piffanelli contributed equally to this work.

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Autor: Magali Lescot - Pietro Piffanelli - Ana Y Ciampi - Manuel Ruiz - Guillaume Blanc - Jim Leebens-Mack - Felipe R da Silva -


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