Alterations in Sensitivity to Estrogen, Dihydrotestosterone, and Xenogens in B-Lymphocytes from Children with Autism Spectrum Disorder and Their Unaffected Twins-SiblingsReportar como inadecuado




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Journal of ToxicologyVolume 2013 2013, Article ID 159810, 11 pages

Research ArticleDepartment of Neurosurgery, The Methodist Neurological Institute, 6560 Fannin Street, Scurlock Tower 944, Houston, TX 77030, USA

Received 28 March 2013; Revised 17 September 2013; Accepted 17 September 2013

Academic Editor: Cinta Porte

Copyright © 2013 Martyn A. Sharpe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

It has been postulated that androgen overexposure in a susceptible person leads to excessive brain masculinization and the autism spectrum disorder ASD phenotype. In this study, the responses to estradiol E2, dihydrotestosterone DHT, and dichlorodiphenyldichloroethylene DDE on B-lymphocytes from ASD subjects and controls are compared. B cells were obtained from 11 ASD subjects, their unaffected fraternal twins, and nontwin siblings. Controls were obtained from a different cell bank. Lactate dehydrogenase LDH and sodium 2,3-bis2-methoxy-4-nitro-5-sulfophenyl-2H-tetrazolium-5-carboxanilide XTT reduction levels were measured after incubation with different concentrations of E2, DHT, and DDE. XTT-LDH ratio, representative of mitochondria number per cell, was calculated. E2, DHT, and DDE all cause “U”-shaped growth curves, as measured by LDH levels. ASD B cells show less growth depression compared to siblings and controls . They also have reduced XTT-LDH ratios when compared to external controls, whereas siblings had values of XTT-LDH between ASD and external controls. B-lymphocytes from people with ASD exhibit a differential response to E2, DHT, and hormone disruptors in regard to cell growth and mitochondrial upregulation when compared to non-ASD siblings and external controls. Specifically, ASD B-lymphocytes show significantly less growth depression and less mitochondrial upregulation when exposed to these effectors. A mitochondrial deficit in ASD individuals is implied.





Autor: Martyn A. Sharpe, Taylor L. Gist, and David S. Baskin

Fuente: https://www.hindawi.com/



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