Ability of Lactobacillus fermentum to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharidesReportar como inadecuado




Ability of Lactobacillus fermentum to overcome host α-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya α-galacto-oligosaccharides - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Microbiology

, 8:22

First Online: 29 January 2008Received: 26 October 2007Accepted: 29 January 2008

Abstract

BackgroundSoya and its derivatives represent nutritionally high quality food products whose major drawback is their high content of α-galacto-oligosaccharides. These are not digested in the small intestine due to the natural absence of tissular α-galactosidase in mammals. The passage of these carbohydrates to the large intestine makes them available for fermentation by gas-producing bacteria leading to intestinal flatulence. The aim of the work reported here was to assess the ability of α-galactosidase-producing lactobacilli to improve the digestibility of α-galacto-oligosaccharides in situ.

ResultsGnotobiotic rats were orally fed with soy milk and placed in respiratory chambers designed to monitor fermentative gas excretion. The validity of the animal model was first checked using gnotobiotic rats monoassociated with a Clostridium butyricum hydrogen H2-producing strain. Ingestion of native soy milk by these rats caused significant H2 emission while ingestion of α-galacto-oligosaccharide-free soy milk did not, thus validating the experimental system. When native soy milk was fermented using the α-galactosidase-producing Lactobacillus fermentum CRL722 strain, the resulting product failed to induce H2 emission in rats thus validating the bacterial model. When L. fermentum CRL722 was coadministered with native soy milk, a significant reduction 50 %, P = 0.019 in H2 emission was observed, showing that α-galactosidase from L. fermentum CRL722 remained active in situ, in the gastrointestinal tract of rats monoassociated with C. butyricum. In human-microbiota associated rats, L. fermentum CRL722 also induced a significant reduction of H2 emission 70 %, P = 0.004.

ConclusionThese results strongly suggest that L. fermentum α-galactosidase is able to partially alleviate α-galactosidase deficiency in rats. This offers interesting perspectives in various applications in which lactic acid bacteria could be used as a vector for delivery of digestive enzymes in man and animals.

Abbreviationsα-Galα-galactosidase

α-GOSα-galacto-oligosaccharides

BHIBrain heart infusion

CbClostridium butyricum

CFUColony-forming unit

HMAHuman microbiota-associated

LABLactic acid bacteria

MRSMan Rogosa Sharp

MWMetabolic weight

pNPGp-nitrophenyl-α-D-galactopyranoside

X-α-Gal5-Bromo-4-chloro-3-indolyl α-D-galactopyranoside.

Jean Guy LeBlanc, Florence Ledue-Clier contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2180-8-22 contains supplementary material, which is available to authorized users.

Jean Guy LeBlanc, Florence Ledue-Clier contributed equally to this work.

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Autor: Jean Guy LeBlanc - Florence Ledue-Clier - Martine Bensaada - Graciela Savoy de Giori - Theodora Guerekobaya - Fernando Ses

Fuente: https://link.springer.com/article/10.1186/1471-2180-8-22



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