Identification of a subunit of NADH-dehydrogenase as a p49-STRAP-binding proteinReportar como inadecuado




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BMC Cell Biology

, 9:8

First Online: 29 January 2008Received: 01 August 2007Accepted: 29 January 2008

Abstract

BackgroundThe p49-STRAP or SRFBP1 protein was recently identified in our laboratory as a cofactor of serum response factor that contributes to the regulation of SRF target genes in the heart.

ResultsIn the present study, we report that NDUFAB1, a nuclear encoded subunit of NADH dehydrogenase, represented the majority of the cDNA clones that interacted with p49-STRAP in multiple screenings using the yeast two-hybrid system. The p49-STRAP and NDUFAB1 proteins interacted and co-localized with each other in the cell. The p49-STRAP protein contains four classic nuclear localization sequence motifs, and it was observed to be present predominantly in the nucleus. Overexpression of p49-STRAP altered the intracellular level of NAD, and reduced the NAD-NADH ratio. Overexpression of p49-STRAP also induced the deacetylation of serum response factor.

ConclusionThese data suggest that p49-STRAP plays a role in the regulation of intracellular processes such as cardiac cellular metabolism, gene expression, and possibly aging.

AbbreviationsNADHNicotinamide adenine dinucleotide hydride

NADNicotinamide adenine dinucleotide

p49-STRAP-SRF-dependent transcription regulation-associated protein- which is a 49-kilodalton protein

SRFBP1-serum response factor binding protein 1- which is a synonym of p49-STRAP

NDUFAB1NADH dehydrogenase ubiquinone 1, alpha-beta subcomplex, 1, 8 kDa

HAHemagglutinin

SRFSerum response factor

Zipzap-p200-Zinc finger protein with activation potential- which is 200-kilodalton protein

EGFPEnhanced green fluorescent protein

MOIMultiplicity of infection

NLSNuclear localization sequence

pat4Pattern-4

pat7Pattern-7.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2121-9-8 contains supplementary material, which is available to authorized users.

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Autor: Xiaomin Zhang - Gohar Azhar - Scott Helms - Ying Zhong - Jeanne Y Wei

Fuente: https://link.springer.com/article/10.1186/1471-2121-9-8







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