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BMC Genomics

, 9:52

First Online: 29 January 2008Received: 30 October 2007Accepted: 29 January 2008

Abstract

BackgroundMicroRNAs miRNAs are a novel class of gene expression regulators implicated in cancer biology. Neuroblastoma NB is an embryonal tumour consisting of neural crest-derived undifferentiated cells and is characterised by variable clinical courses ranging from spontaneous regression to therapy-resistant progression. Recent advances identified a subset of miRNAs with putative function in NB biology. However, the full repertoire of miRNAs expressed in NBs is not available.

ResultsWe describe miRNA profiles of 13 NB specimens and 2 NB cell lines as determined by miRNA cloning. A total of 3153 sequences were sequenced and analysed by a miRNA prediction tool miRpredict. Our library covered 27% miRNAs known to date. 39 reads corresponding to 25 individual sequences were classified as novel miRNAs, including miRNA* species of 10 known miRNAs. Expression of 5 new miRNA* forms and 8 individual sequences was supported by Northern blotting. Most of the novel miRNA genes are not related to each other and do not share homology with the annotated sequences in the public miRNA database, but they are conserved within mammals or have close homologues in primates genomes.

ConclusionWe provide evidence for 29 new miRNA and miRNA-like sequences 24 novel sequences and 5 miRNAs discovered initially in other species. Some of these newly identified sequences reside within frequently altered chromosomal regions in NB tumours and may play a role in NB biology.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-9-52 contains supplementary material, which is available to authorized users.

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Autor: Elena A Afanasyeva - Agnes Hotz-Wagenblatt - Karl-Heinz Glatting - Frank Westermann

Fuente: https://link.springer.com/article/10.1186/1471-2164-9-52



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