Bunched, the Drosophilahomolog of the mammalian tumor suppressor TSC-22, promotes cellular growthReportar como inadecuado




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BMC Developmental Biology

, 8:10

First Online: 28 January 2008Received: 27 November 2007Accepted: 28 January 2008

Abstract

BackgroundTransforming Growth Factor-β1 stimulated clone-22 TSC-22 is assumed to act as a negative growth regulator and tumor suppressor. TSC-22 belongs to a family of putative transcription factors encoded by four distinct loci in mammals. Possible redundancy among the members of the TSC-22-Dip-Bun protein family complicates a genetic analysis. In Drosophila, all proteins homologous to the TSC-22-Dip-Bun family members are derived from a single locus called bunched bun.

ResultsWe have identified bun in an unbiased genetic screen for growth regulators in Drosophila. Rather unexpectedly, bun mutations result in a growth deficit. Under standard conditions, only the long protein isoform BunA – but not the short isoforms BunB and BunC – is essential and affects growth. Whereas reducing bunA function diminishes cell number and cell size, overexpression of the short isoforms BunB and BunC antagonizes bunA function.

ConclusionOur findings establish a growth-promoting function of Drosophila BunA. Since the published studies on mammalian systems have largely neglected the long TSC-22 protein version, we hypothesize that the long TSC-22 protein is a functional homolog of BunA in growth regulation, and that it is antagonized by the short TSC-22 protein.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-8-10 contains supplementary material, which is available to authorized users.

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Autor: Silvia Gluderer - Sean Oldham - Felix Rintelen - Andrea Sulzer - Corina Schütt - Xiaodong Wu - Laurel A Raftery - Ernst 

Fuente: https://link.springer.com/article/10.1186/1471-213X-8-10







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