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BMC Developmental Biology

, 7:9

First Online: 17 February 2007Received: 23 June 2006Accepted: 17 February 2007

Abstract

BackgroundInterfollicular skin develops normally only when the activity of the progenitor cells in the basal layer is counterbalanced by the exit of cells into the suprabasal layers, where they differentiate and cornify to establish barrier function. Distinct stem and progenitor compartments have been demonstrated in hair follicles and sebaceous glands, but there are few data to describe the control of interfollicular progenitor cell activity. Wnt signaling has been shown to be an important growth-inducer of stem cell compartments in skin and many other tissues.

ResultsHere, we test the effect of ectopic Wnt1 expression on the behavior of interfollicular progenitor cells in an organotypic culture model, and find that Wnt1 signaling inhibits their growth and promotes terminal differentiation.

ConclusionThese results are consistent with the phenotypes reported for transgenic mice engineered to have gain or loss of function of Wnt signaling in skin, which would recommend our culture model as an accurate one for molecular analysis. Since it is known that canonical ligands are expressed in skin, it is likely that this pathway normally regulates the balance of growth and differentiation, and suggests it could be important to pathogenesis.

AbbreviationsNIKSNear-diploid Immortal Keratinocytes

Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-7-9 contains supplementary material, which is available to authorized users.

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Autor: Marni A Slavik - B Lynn Allen-Hoffmann - Bob Y Liu - Caroline M Alexander

Fuente: https://link.springer.com/article/10.1186/1471-213X-7-9







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