High resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivoin miceReportar como inadecuado




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BMC Developmental Biology

, 6:10

First Online: 27 February 2006Received: 08 August 2005Accepted: 27 February 2006

Abstract

BackgroundIn utero microinjection has proven valuable for exploring the developmental consequences of altering gene expression, and for studying cell lineage or migration during the latter half of embryonic mouse development from embryonic day 9.5 of gestation E9.5. In the current study, we use ultrasound guidance to accurately target microinjections in the conceptus at E6.5–E7.5, which is prior to cardiovascular or placental dependence. This method may be useful for determining the developmental effects of targeted genetic or cellular interventions at critical stages of placentation, gastrulation, axis formation, and neural tube closure.

ResultsIn 40 MHz ultrasound images at E6.5, the ectoplacental cone region and proamniotic cavity could be visualized. The ectoplacental cone region was successfully targeted with 13.8 nL of a fluorescent bead suspension with few or no beads off-target in 51% of concepti microinjected at E6.5 28-55 injected. Seventy eight percent of the embryos survived 2 to 12 days post injection 93-119, 73% 41-56 survived to term of which 68% 38-56 survived and appeared normal one week after birth. At E7.5, the amniotic and exocoelomic cavities, and ectoplacental cone region were discernable. Our success at targeting with few or no beads off-target was 90% 36-40 for the ectoplacental cone region and 81% 35-43 for the exocoelomic cavity but tended to be less, 68% 34-50, for the smaller amniotic cavity. At E11.5, beads microinjected at E7.5 into the ectoplacental cone region were found in the placental spongiotrophoblast layer, those injected into the exocoelomic cavity were found on the surface or within the placental labyrinth, and those injected into the amniotic cavity were found on the surface or within the embryo. Following microinjection at E7.5, survival one week after birth was 60% 26-43 when the amniotic cavity was the target and 66% 19-29 when the target was the ectoplacental cone region. The survival rate was similar in sham experiments, 54% 33-61, for which procedures were identical but no microinjection was performed, suggesting that surgery and manipulation of the uterus were the main causes of embryonic death.

ConclusionUltrasound-guided microinjection into the ectoplacental cone region at E6.5 or E7.5 and the amniotic cavity at E7.5 was achieved with a 7 day postnatal survival of ≥60%. Target accuracy of these sites and of the exocoelomic cavity at E7.5 was ≥51%. We suggest that this approach may be useful for exploring gene function during early placental and embryonic development.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-213X-6-10 contains supplementary material, which is available to authorized users.

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Autor: John C Slevin - Lois Byers - Marina Gertsenstein - Dawei Qu - Junwu Mu - Nana Sunn - John CP Kingdom - Janet Rossant - S

Fuente: https://link.springer.com/article/10.1186/1471-213X-6-10







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