Bioconversion of Airborne Methylamine by Immobilized Recombinant Amine Oxidase from the Thermotolerant Yeast Hansenula polymorphaReportar como inadecuado




Bioconversion of Airborne Methylamine by Immobilized Recombinant Amine Oxidase from the Thermotolerant Yeast Hansenula polymorpha - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

The Scientific World Journal - Volume 2014 2014, Article ID 898323, 9 pages -

Research Article

Department of Chemical Engineering and Biotechnology, Ariel University, 40700 Ariel, Israel

The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, 52900 Ramat Gan, Israel

Department of Analytical Biotechnology, Institute of Cell Biology, National Academy of Science of Ukraine, Drahomanov Street 14-16, Lviv 79005, Ukraine

Institute of Applied Biotechnology and Basic Sciences, University of Rzeszow, Sokolowska Street 26, 36-100 Kolbuszowa, Poland

Received 29 August 2013; Accepted 26 October 2013; Published 29 January 2014

Academic Editors: F. Long and Y. Ueta

Copyright © 2014 Sasi Sigawi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aliphatic amines, including methylamine, are air-pollutants, due to their intensive use in industry and the natural degradation of proteins, amino acids, and other nitrogen-containing compounds in biological samples. It is necessary to develop systems for removal of methylamine from the air, since airborne methylamine has a negative effect on human health. The primary amine oxidase primary amine : oxygen oxidoreductase deaminating or amine oxidase, AMO; EC 1.4.3.21, a copper-containing enzyme from the thermotolerant yeast Hansenula polymorpha which was overexpressed in baker’s yeast Saccharomyces cerevisiae, was tested for its ability to oxidize airborne methylamine. A continuous fluidized bed bioreactor CFBR was designed to enable bioconversion of airborne methylamine by AMO immobilized in calcium alginate CA beads. The results demonstrated that the bioreactor with immobilized AMO eliminates nearly 97% of the airborne methylamine. However, the enzymatic activity of AMO causes formation of formaldehyde. A two-step bioconversion process was therefore proposed. In the first step, airborne methylamine was fed into a CFBR which contained immobilized AMO. In the second step, the gas flow was passed through another CFBR, with alcohol oxidase from the yeast H. polymorpha immobilized in CA, in order to decompose the formaldehyde formed in the first step. The proposed system provided almost total elimination of the airborne methylamine and the formaldehyde.





Autor: Sasi Sigawi, Marina Nisnevitch, Oksana Zakalska, Andriy Zakalskiy, Yeshayahu Nitzan, and Mykhailo Gonchar

Fuente: https://www.hindawi.com/



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