Comparative mapping of expressed sequence tags containing microsatellites in rainbow trout Oncorhynchus mykissReportar como inadecuado




Comparative mapping of expressed sequence tags containing microsatellites in rainbow trout Oncorhynchus mykiss - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Genomics

, 6:54

First Online: 18 April 2005Received: 20 December 2004Accepted: 18 April 2005

Abstract

BackgroundComparative genomics, through the integration of genetic maps from species of interest with whole genome sequences of other species, will facilitate the identification of genes affecting phenotypes of interest. The development of microsatellite markers from expressed sequence tags will serve to increase marker densities on current salmonid genetic maps and initiate in silico comparative maps with species whose genomes have been fully sequenced.

ResultsEighty-nine polymorphic microsatellite markers were generated for rainbow trout of which at least 74 amplify in other salmonids. Fifty-five have been associated with functional annotation and 30 were mapped on existing genetic maps. Homologous sequences were identified for 20 of the EST containing microsatellites to identify comparative assignments within the tetraodon, mouse, and-or human genomes.

ConclusionThe addition of microsatellite markers constructed from expressed sequence tag data will facilitate the development of high-density genetic maps for rainbow trout and comparative maps with other salmonids and better studied species.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-6-54 contains supplementary material, which is available to authorized users.

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Autor: Caird E RexroadIII - Maria F Rodriguez - Issa Coulibaly - Karim Gharbi - Roy G Danzmann - Jenefer DeKoning - Ruth Philli

Fuente: https://link.springer.com/article/10.1186/1471-2164-6-54



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