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BMC Genomics

, 5:46

First Online: 16 July 2004Received: 24 March 2004Accepted: 16 July 2004

Abstract

BackgroundIn mammalian cells changes in intracellular pH pHi, which are predominantly controlled by activity of plasma membrane ion exchangers, regulate a diverse range of normal and pathological cellular processes. How changes in pHi affect distinct cellular processes has primarily been determined by evaluating protein activities and we know little about how pHi regulates gene expression.

ResultsA global profile of genes regulated in mammalian fibroblasts by decreased pHi induced by impaired activity of the plasma membrane Na-H exchanger NHE1 was characterized by using cDNA microarrays. Analysis of selected genes by quantitative RT-PCR, TaqMan, and immunoblot analyses confirmed results obtained from cDNA arrays. Consistent with established roles of pHi and NHE1 activity in cell proliferation and oncogenic transformation, grouping regulated genes into functional categories and biological pathways indicated a predominant number of genes with altered expression were associated with growth factor signaling, oncogenesis, and cell cycle progression.

ConclusionA comprehensive analysis of genes selectively regulated by pHi provides insight on candidate targets that might mediate established effects of pHi on a number of normal and pathological cell functions.

AbbreviationsEzrinradixin, moesin ERM

Intracellular pH pHiNa-H exchanger type 1 NHE1

NGALNeutrophil Gelatinase Associated Lipocalin, type IV collagenase MMP-9.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-5-46 contains supplementary material, which is available to authorized users.

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Autor: Luanna K Putney - Diane L Barber

Fuente: https://link.springer.com/article/10.1186/1471-2164-5-46







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