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BMC Genomics

, 5:42

First Online: 06 July 2004Received: 16 June 2004Accepted: 06 July 2004


BackgroundUnravelling the path from genotype to phenotype, as it is influenced by an organism-s environment, is one of the central goals in biology. Gene expression profiling by means of microarrays has become very prominent in this endeavour, although resources exist only for relatively few model systems. As genomics has matured into a comparative research program, expression profiling now also provides a powerful tool for non-traditional model systems to elucidate the molecular basis of complex traits.

ResultsHere we present a microarray constructed with ~4500 features, derived from a brain-specific cDNA library for the African cichlid fish Astatotilapia burtoni Perciformes. Heterologous hybridization, targeting RNA to an array constructed for a different species, is used for eight different fish species. We quantified the concordance in gene expression profiles across these species number of genes and fold-changes. Although most robust when target RNA is derived from closely related species <10 MA divergence time, our results showed consistent profiles for other closely related taxa ~65 MA divergence time and, to a lesser extent, even very distantly related species >200 MA divergence time.

ConclusionThis strategy overcomes some of the restrictions imposed on model systems that are of importance for evolutionary and ecological studies, but for which only limited sequence information is available. Our work validates the use of expression profiling for functional genomics within a comparative framework and provides a foundation for the molecular and cellular analysis of complex traits in a wide range of organisms.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2164-5-42 contains supplementary material, which is available to authorized users.

Susan CP Renn, Nadia Aubin-Horth contributed equally to this work.

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Autor: Susan CP Renn - Nadia Aubin-Horth - Hans A Hofmann


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