Cytotoxicity of Protein-Carbon Nanotubes on J774 Macrophages Is a Functionalization Grade-Dependent EffectReportar como inadecuado

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BioMed Research International - Volume 2015 2015, Article ID 796456, 8 pages -

Research Article

Centro de Investigación en Materiales Avanzados CIMAV, Miguel de Cervantes 120, Complejo Industrial Chihuahua, 31109 Chihuahua, CHIH, Mexico

Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua, Nuevo Campus Universitario, Circuito No. 1, 31125 Chihuahua, CHIH, Mexico

Coordinación Académica Región Altiplano, Universidad Autónoma de San Luis Potosí, 78000 Matehuala, SLP, Mexico

Received 24 June 2014; Accepted 23 September 2014

Academic Editor: Haseeb A. Khan

Copyright © 2015 Silvia Lorena Montes-Fonseca et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Carbon nanotubes CNTs are used as carriers in medicine due to their ability to be functionalized with chemical substances. However, cytotoxicity analysis is required prior to use for in vivo models. The aim of this study was to evaluate the cytotoxic effect of CNTs functionalized with a 46 kDa surface protein from Entamoeba histolytica P46-CNTs on J774A macrophages. With this purpose, CNTs were synthesized by spray pyrolysis and purified P-CNTs using sonication for 48 h. A 46 kDa protein, with a 4.6–5.4 pI range, was isolated from E. histolytica HM1:IMSS strain trophozoites using an OFFGEL system. The P-CNTs were functionalized with the purified 46 kDa protein, classified according to their degree of functionalization, and characterized by Raman and Infrared spectroscopy. In vitro cytotoxicity was evaluated by MTT, apoptosis, and morphological assays. The results demonstrated that P46-CNTs exhibited cytotoxicity dependent upon the functionalized grade. Contrary to what was expected, P46-CNTs with a high grade of functionalization were more toxic to J774 macrophages than P46-CNTs with a low grade of functionalization, than P-CNTs, and had a similar level of toxicity as UP-CNT. This suggests that the nature of the functionalized protein plays a key role in the cytotoxicity of these nanoparticles.

Autor: Silvia Lorena Montes-Fonseca, Blanca Sánchez-Ramírez, Antonia Luna-Velasco, Carlos Arzate-Quintana, Macrina Beatriz Silva-Ca



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