Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates.Reportar como inadecuado




Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

* Corresponding author 1 Department of Engineering Science 2 Department of Physiology, Anatomy and Genetics 3 LIPhy - Laboratoire Interdisciplinaire de Physique Saint Martin d’Hères

Abstract : Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.





Autor: Edward J Botcherby - Christopher W Smith - Michael M Kohl - Delphine Débarre - Martin J Booth - Rimas Juskaitis - Ole Paulsen -

Fuente: https://hal.archives-ouvertes.fr/



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