Conjugation of E. coli O157:H7 Antibody to CdSe-ZnS Quantum DotsReportar como inadecuado




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Journal of NanomaterialsVolume 2015 2015, Article ID 265315, 7 pages

Research ArticleUniversity of Science, Vietnam National University-Ho Chi Minh City, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City 749000, Vietnam

Received 15 September 2015; Accepted 16 November 2015

Academic Editor: Daniela Predoi

Copyright © 2015 N. T. Vo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The conjugation of antibody to semiconductor quantum dots plays a very important role in many applications such as bioimaging, biomarking, and biosensing. In this research, we present some results of highly luminescent core-shell structure CdSe-ZnS on which the E. coli antibody was conjugated. The CdSe core was synthesized successfully with chemical “green” method. For biological applications, the capping surfactant, trioctylphosphine oxide, was substituted by a new one, mercaptopropionic acid MPA, before the antibody attachment step. Finally, the E. coli antibody was attached to quantum dots CdSe-ZnS. Morphology, structure, and optical properties were investigated with PL, UV-Vis, TEM, and XRD methods. The successful ligand substitution and antibody attachment were confirmed by zeta potential measurement, FTIR spectroscopy, and TEM. The results showed quantum dots size of 2.3 nm, uniform distribution, and high luminescence. CdSe-ZnS core-shell structure had better stability and enhanced the luminescence efficiency up to threefold compared with the core CdSe. MPA ligand shifted the initial hydrophobic quantum dots to hydrophilic ones, which helped to dissolve them in organic solvents and attach the antibody.





Autor: N. T. Vo, H. D. Ngo, D. L. Vu, A. P. Duong, and Q. V. Lam

Fuente: https://www.hindawi.com/



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