Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus Reportar como inadecuado

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1 Trivedi Global Inc. 2 Trivedi science research laboratory pvt. Ltd.

Abstract : Staphylococcus aureus S. aureus is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on S. aureus. S. aureus ATCC 25923 was divided into two parts, Group Gr. I: control and Gr. II: treatment. After biofield treatment, Gr. II was further subdivided into two parts, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 159 after revival Study I. The revived sample Gr. IIB were retreated on day 159 Study II, and divided into three separate tubes. Tube 1 was analyzed on day 5, likewise, tube 2 and 3 were analyzed on day 10 and 15, respectively. All the experimental parameters were studied using automated MicroScan Walk-Away® system. The 16S rDNA sequencing was carried out in Gr. IIA sample to correlate the phylogenetic relationship of S. aureus with other bacterial species. The antimicrobial susceptibility and minimum inhibitory concentration showed significant alteration i.e. 92.86% and 90.00% respectively in treated cells of S. aureus as compared to control. The biochemical reactions also showed the significant 35.71% alteration in treated sample with respect to control. The biotype number and microbial species were substantially changed in Gr. IIA 767177; Staphylococcus cohnii subsp. urealyticum on day 10, while only the biotype numbers were changed in rest of the treated samples as compared to control 307016; S. aureus. The 16S rDNA analysis showed that the identified strain in this experiment was S. aureus GenBank Accession No.: L37597 after biofield treatment. However, the nearest homolog genus-species was found as Staphylococcus simiae GenBank Accession No.: DQ127902. These results suggested that biofield treatment has a significant impact on S. aureus in lyophilized as well as revived state.

Keywords : Staphylococcus Aureus Antibiogram Staphylococci Staphylococcus Aureus Antimicrobial Sensitivity Biofield Treatment Biochemical Reaction Biotype 16s rDNA Gram-Positive Bacteria Trivedi Effect Staphylococcus Aureus Characteristics

Autor: Mahendra Kumar Trivedi - Alice Branton - Dahryn Trivedi - Gopal Nayak - Sambhu Charan Mondal - Snehasis Jana -



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