Curative Effects of Oleanolic Acid on Formed Hypertrophic Scars in the Rabbit Ear ModelReportar como inadecuado

Curative Effects of Oleanolic Acid on Formed Hypertrophic Scars in the Rabbit Ear Model - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Evidence-Based Complementary and Alternative MedicineVolume 2012 2012, Article ID 837581, 10 pages

Research Article

Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Key Laboratory of Standardization of Chinese Herbal Medicines of Ministry of Education, Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China

Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China

Shanghai Anshan Experimental Middle School, Shanghai 200433, China

The School of Pharmacy & Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, Liverpool L3 3AF, UK

Received 9 November 2012; Revised 11 December 2012; Accepted 11 December 2012

Academic Editor: Ke Liu

Copyright © 2012 Hong Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Hypertrophic scarring is a common proliferative disorder of dermal fibroblasts characterized by collagen overproduction and excessive deposition of extracellular matrix ECM. There is no consensus about the best therapeutics to produce complete and permanent improvement of scars with few side effects. To investigate the therapeutic effects of oleanolic acid OA on hypertrophic scars and explore the possible mechanism of action involved, a rabbit ear model with hypertrophic scars was established. OA 2.5%, 5%, and 10% was given once daily to the scars for 28 consecutive days. As a result, OA significantly alleviated formed hypertrophic scars on rabbit ears. The levels of TGF- , MMP-1, TIMP-1, and collagens I and III were notably decreased, and the number of apoptosis cells and mRNA expression of MMP-2, caspase-3, and caspase-9 were markedly increased in the scar tissue. The scar elevation index SEI was also evidently reduced. Histological findings exhibited significant amelioration of the collagen tissue. These results suggest that OA has the favorable curative effects on formed hypertrophic scars in the rabbit ear model, and the possible mechanism of action is that OA decreases HSFs proliferation and increases HSFs apoptosis by reduction of P311 gene expression and TGF- production, inhibition of TIMP-1 secretion, enhancement of MMP-2 activity, and subsequently facilitation of degradation of collagen types I and III.

Autor: Hong Zhang, Yan Zhang, Yi-Ping Jiang, Lan-Ke Zhang, Cheng Peng, Kun He, Khalid Rahman, and Lu-Ping Qin



Documentos relacionados