Serial increase of IL-12 response and human leukocyte antigen-DR expression in severe sepsis survivorsReportar como inadecuado




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Critical Care

, 15:R224

First Online: 22 September 2011Received: 25 April 2011Revised: 05 August 2011Accepted: 22 September 2011

Abstract

IntroductionSepsis-induced immunosuppression may result in death. The mechanisms of immune suppression include loss of macrophage and monocyte expression of the major histocompatibility complex, increased anti-inflammatory cytokine expression and decreased expression of proinflammatory cytokines. In this study, we sought to determine the mechanisms of immune suppression in severe sepsis by repeated detection.

MethodsWe designed this prospective observational study to measure monocyte human leukocyte antigen HLA-DR expression, plasma cytokine levels and cytokine responses on days 1 and 7 in stimulated peripheral blood mononuclear cells PBMCs of healthy controls and patients with severe sepsis.

ResultsOf the 35 enrolled patients, 23 survived for 28 days and 12 died, 6 of whom died within 7 days. Plasma levels of IL-1β, IL-6, IL-10, IL-17, transforming growth factor TGF-β1 and TNF-α were higher, but plasma IL-12 level was lower in septic patients than those in controls. Day 1 plasma levels of IL-1β, IL-6, IL-10 and TGF-β1 in nonsurvivors were higher than those in survivors. Day 7 plasma IL-10 levels in nonsurvivors were higher than in survivors. IL-1β response was higher, but IL-12 and TNF-α responses were lower in septic patients than in controls. Day 1 IL-6 response was lower, but day 1 TGF-β1 response was higher in nonsurvivors than in survivors. Plasma IL-6 and IL-10 levels were decreased in survivors after 6 days. IL-6 response was decreased in survivors after 6 days, but IL-12 response was increased. Monocyte percentage was higher, but positive HLA-DR percentage in monocytes and mean fluorescence intensity MFI of HLA-DR were lower in septic patients than in controls. MFI of HLA-DR was increased in survivors after 6 days.

ConclusionsMonocyte HLA-DR expression and IL-12 response from PBMCs are restored in patients who survive severe sepsis.

Keywordsinterleukin 12 interleukin 6 human leukocyte antigen-DR peripheral blood mononuclear cells severe sepsis AbbreviationsAPACHE IIAcute Physiology and Chronic Health Evaluation II

BSAbovine serum albumin

ELISAenzyme-linked immunosorbent assay

HLAhuman leukocyte antigen

ILinterleukin

LPSlipopolysaccharide

MFImeans of fluorescence intensity

NKnatural killer cell

PBMCperipheral blood mononuclear cell

PBSphosphate-buffered saline

SIRSsystemic inflammatory response syndrome

SPSSStatistical Package for the Social Sciences

ThT helper cell

TGFtransforming growth factor

TNFtumor necrosis factor.

Electronic supplementary materialThe online version of this article doi:10.1186-cc10464 contains supplementary material, which is available to authorized users.

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Autor: Huang-Pin Wu - Chi-Chung Shih - Chun-Yao Lin - Chung-Ching Hua - Duen-Yau Chuang

Fuente: https://link.springer.com/







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