Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal BacteremiaReportar como inadecuado




Usefulness of Multiplex Real-Time PCR for Simultaneous Pathogen Detection and Resistance Profiling of Staphylococcal Bacteremia - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BioMed Research International - Volume 2016 2016, Article ID 6913860, 7 pages -

Research Article

Department of Laboratory Medicine, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea

Department of Laboratory Medicine, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea

Department of Laboratory Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Republic of Korea

Department of Internal Medicine, Seoul National University Bundang Hospital, 82 Gumi-ro, 173 Beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13620, Republic of Korea

Received 24 March 2016; Accepted 23 May 2016

Academic Editor: Giulio Mengozzi

Copyright © 2016 Yousun Chung et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Staphylococci are the leading cause of nosocomial blood stream infections. Fast and accurate identification of staphylococci and confirmation of their methicillin resistance are crucial for immediate treatment with effective antibiotics. A multiplex real-time PCR assay that targets mecA, femA specific for S. aureus, femA specific for S. epidermidis, 16S rRNA for universal bacteria, and 16S rRNA specific for staphylococci was developed and evaluated with 290 clinical blood culture samples containing Gram-positive cocci in clusters GPCC. For the 262 blood cultures identified to the species level with the MicroScan WalkAway system Siemens Healthcare Diagnostics, USA, the direct real-time PCR assay of positive blood cultures showed very good agreement for the categorization of staphylococci into methicillin-resistant S. aureus MRSA, methicillin-susceptible S. aureus MSSA, methicillin-resistant S. epidermidis MRSE, methicillin-susceptible S. epidermidis MSSE, methicillin-resistant non-S. epidermidis CoNS MRCoNS, and methicillin-susceptible non-S. epidermidis CoNS MSCoNS . The direct multiplex real-time PCR assay of positive blood cultures containing GPCC can provide essential information at the critical point of infection with a turnaround time of no more than 4 h. Further studies should evaluate the clinical outcome of using this rapid real-time PCR assay in glycopeptide antibiotic therapy in clinical settings.





Autor: Yousun Chung, Taek Soo Kim, Young Gi Min, Yun Ji Hong, Jeong Su Park, Sang Mee Hwang, Kyoung-Ho Song, Eu Suk Kim, Kyoung U

Fuente: https://www.hindawi.com/



DESCARGAR PDF




Documentos relacionados