Immunomodulation by fish-oil containing lipid emulsions in murine acute respiratory distress syndromeReportar como inadecuado

Immunomodulation by fish-oil containing lipid emulsions in murine acute respiratory distress syndrome - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Critical Care

, 18:R85

First Online: 29 April 2014Received: 04 October 2013Accepted: 14 January 2014


IntroductionAcute respiratory distress syndrome ARDS is a major cause of mortality in intensive care units. Patients with ARDS often require parenteral nutrition with lipid emulsions as essential components. Besides being an energy supply, these lipid emulsions might display differential modulatory effects on lung integrity and inflammation.

MethodsIn a pre-emptive strategy, we investigated the impact of three different intravenously infused lipid emulsions on lung morphology, leukocyte invasion, protein leakage and cytokines in a murine model of ARDS. Mice received an infusion of normal saline solution, a pure long-chain triglycerides LCT emulsion, a medium-chain triglycerides MCT containing mixed emulsion LCT-MCT, or a fish oil FO containing mixed emulsion LCT-MCT-FO before lipopolysaccharide LPS challenge.

ResultsMice pre-infused with fish oil-containing lipid emulsion showed decreased leukocyte invasion, protein leakage, myeloperoxidase activity, and cytokine production in their alveolar space after LPS challenge compared to mice receiving LCT or LCT-MCT. In line with these findings, lung morphology assessed by histological staining after LPS-induced lung injury improved faster in the LCT-MCT-FO group. Concerning the above mentioned parameters, no significant difference was observed between mice infused with LCT or the combination of LCT and MCT.

ConclusionFish oil-containing lipid emulsions might exert anti-inflammatory and pro-resolving effects in the murine model of acute lung injury. Partial replacement of n-6 fatty acids with n-3 fatty acids may thus be of benefit for critically ill patients at risk for ARDS which require parenteral nutrition.

AbbreviationsAAArachidonic acid

ANOVAAnalysis of variance

ARDSAcute respiratory distress syndrome

BALBronchoalveolar lavage

DHADocosahexaenoic acid

ELISAEnzyme-linked immunosorbent assay

EPAEicosapentaenoic acid

FOFish oil

ICUIntensive care unit

LCTLong chain triglycerides

LOALinoleic acid

LPSLipopolysaccharide, endotoxin

MCTMedium chain trigycerides

MIPMacrophage inflammatory protein



NFNuclear factor

OAOleic acid



PUFAPolyunsaturated fatty acids

SEMStandard error of the mean

TNFTumor-necrosis factor


Electronic supplementary materialThe online version of this article doi:10.1186-cc13850 contains supplementary material, which is available to authorized users.

Matthias Hecker, Juliane Ott contributed equally to this work.

Download fulltext PDF

Autor: Matthias Hecker - Juliane Ott - Christoph Sondermann - Martina Barbara Schaefer - Martin Obert - Andreas Hecker - Rory E 


Documentos relacionados