The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical AnalysisReportar como inadecuado

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Evidence-Based Complementary and Alternative MedicineVolume 2012 2012, Article ID 508972, 15 pages

Research Article

Institute of Biochemistry and Molecular Biology, School of Life Sciences, National Yang-Ming University, Taipei 11221, Taiwan

Institute of Traditional Medicine, National Yang-Ming University, Taipei 11221, Taiwan

Department of Education and Research, Taipei City Hospital, Taipei 11221, Taiwan

Department of Medicine, Mackay Medical College, Taipei 25245, Taiwan

Received 27 May 2012; Revised 16 July 2012; Accepted 18 July 2012

Academic Editor: Yoshiyuki Kimura

Copyright © 2012 Chia-Yen Hsiao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Lithospermi Radix LR is an effective traditional Chinese herb in various types of wound healing; however, its mechanism of action remains unknown. A biochemical and proteomic platform was generated to explore the biological phenomena associated with LR and its active component shikonin. We found that both LR ethanol extracts and shikonin are able to promote cell proliferation by up to 25%. The results of proteomic analysis revealed that twenty-two differentially expressed proteins could be identified when fibroblast cells were treated with LR or shikonin. The functions of those proteins are associated with antioxidant activity, antiapoptosis activity, the regulation of cell mobility, the secretion of collagen, the removal of abnormal proteins, and the promotion of cell proliferation, indicating that the efficacy of LR in wound healing may be derived from a synergistic effect on a number of factors induced by the herbal medicine. Furthermore, an animal model confirmed that LR is able to accelerate wound healing on the flank back of the SD rats. Together these findings help to pinpoint the molecular basis of wound healing process induced by LR.

Autor: Chia-Yen Hsiao, Tung-Hu Tsai, and Kin-Fu Chak



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