Clinical significance of mTOR, ZEB1, ROCK1 expression in lung tissues of pulmonary fibrosis patientsReport as inadecuate

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BMC Pulmonary Medicine

, 14:168

Infectious, Rare and Idiopathic Pulmonary Diseases


BackgroundIdiopathic pulmonary fibrosis IPF is a fatal lung disease of unknown causes. Three proteins mammalian target of rapamycin, mTOR; zinc finger E-box-binding homeobox 1, ZEB1; Rho-associated, coiled-coil containing protein kinase 1, ROCK1 may be related to pulmonary fibrosis. However, they have not been assessed in human pulmonary fibrosis. We assessed the clinical significance of mTOR, ZEB1, and ROCK1 expression in human pulmonary fibrosis of usual interstitial pneumonia UIP pattern.

MethodsThe mTOR, ZEB1, and ROCK1 expression was evaluated by immunohistochemical staining of 30 surgical lung biopsy tissues from 26 IPF and 4 UIP pattern connective tissue disease related interstitial lung disease CTD-ILD patients. The expression scores correlated with the clinical features.

ResultsThe mTOR, ZEB1 and ROCK1 mainly expressed in alveolar epithelial cells of UIP lungs. The histological fibrosis scores and lung function decline in the strong mTOR expression group were higher than those in the weak and intermediate expression group. Patients with positive ZEB1 expression had higher fibrosis scores and greater decline in carbon monoxide diffusion capacity DLCO than patients with negative ZEB1 expression. Patients with positive mTOR or ZEB1 expression had poorer prognosis than that of patients with negative mTOR or ZEB1 expression, although it was not statistically significant. ROCK1 was not associated with the studied clinicopathological features.

ConclusionsThe mTOR and ZEB1 expression in pulmonary fibrosis patients significantly correlated with the fibrosis score and lung function decline, indicating that it may be related to the prognosis of pulmonary fibrosis. Further studies involving large numbers of homogeneous IPF patients are warranted.

KeywordsPulmonary fibrosis Immunohistochemical analysis Usual interstitial pneumonia mTOR ZEB1 ROCK1 Survival AbbreviationsmTORmammalian target of rapamycin

ROCK1Rho-associated, coiled-coil containing protein kinase 1

UIPUsual interstitial pneumonia

ZEB1Zinc finger E-box-binding homeobox 1.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2466-14-168 contains supplementary material, which is available to authorized users.

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Author: Jong Sun Park - Hyo Jin Park - Young Sik Park - Sang-Min Lee - Jae-Joon Yim - Chul-Gyu Yoo - Sung Koo Han - Young Whan


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