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E-Journal of Chemistry - Volume 2 2005, Issue 2, Pages 126-130

Varun Herbals, 5-8-293-A, Mahesh Nagar, Chirag Ali Lane, Hyderabad - 500 001, India

Received 14 January 2005; Accepted 9 March 2005

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present paper deals with the standardization study of pippalyadiyoga powder. It is used as a long acting contraceptive. The standardization of compound drug has been achieved by physico-chemical analysis and high performance liquid chromatography HPLC fingerprint studies. Quantitative evaluation of borax in pippalyadiyoga showed 19.08% as sodium borate. RP-HPLC was performed using methanol and water as mobile phase. The detection and quantification was performed at a wavelength of 345 nm. Linearity of detector response for piperine was between the concentrations 0.005% to 0.1%. The correlation coefficient obtained for the linearity was 0.998. The recovery value of standard piperine was 99.4%. Low value of standard deviation and coefficient of variation are indicative of high precision of the method. Quantitative evaluation of piperine in pippalyadiyoga was found to be 0.339%.





Author: D. Shaila, M. K. Santosh, Biswajit Chowdhury, I. Rajyalakshmi, and I. Sanjeevarao

Source: https://www.hindawi.com/



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