Association of Single Nucleotide Polymorphisms in CYP1B1 and COMT Genes with Breast Cancer Susceptibility in Indian WomenReport as inadecuate




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Disease Markers - Volume 27 2009, Issue 5, Pages 203-210



Indian Institute of Toxicology Research Council of Scientific and Industrial Research, Lucknow- 226 001, India

Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow- 226 014, India

Banaras Hindu University, Varanasi-221 005, India

Received 18 December 2009; Accepted 18 December 2009

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cytochrome P450 1B1 CYP1B1 and catechol-$O$-methyltransferase COMT enzymes play critical roles in estrogen metabolism. Alterations in the catalytic activity of CYP1B1 and COMT enzymes have been found associated with altered breast cancer risk in postmenopausal women in many populations. The substitution of leucine Leu to valine Val at codon 432 increases the catalytic activity of CYP1B1, however, substitution of Val to methionine Met at codon 158 decreases the catalytic activity of COMT. The present study was performed to evaluate the associations of CYP1B1 Leu

Val and-or COMT Val

Met polymorphisms with total, premenopausal and postmenopausal breast cancer risks in Indian women. COMT and CYP1B1 polymorphisms in controls and breast cancer patients were analyzed employing polymerase chain reaction-restriction fragment length polymorphism PCR-RFLP followed by gel electrophoresis. Although CYP1B1 and COMT genotypes did not exhibit statistically significant association with breast cancer risks when analyzed individually, COMT wild type Val

Val in combination with CYP1B1 heterozygous variant Leu

Val OR: 0.21; 95% CI 0.05–0.82, p value; 0.021 and COMT heterozygous variant Val

Met in combination with CYP1B1 wild type Leu

Leu OR: 0.29; 95% CI 0.08–0.96, p value; 0.042 showed significant protective association with premenopausal breast cancer risk. The results demonstrate that CYP1B1 wild type in combination with COMT heterozygous or their inverse combination offer protection against breast cancer in premenopausal Indian women.





Author: Sharawan Yadav, Naveen Kumar Singhal, Virendra Singh, Neeraj Rastogi, Pramod Kumar Srivastava, and Mahendra Pratap Singh

Source: https://www.hindawi.com/



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