Utilization of Crude Glycerol as a Substrate for the Production of Rhamnolipid by Pseudomonas aeruginosaReport as inadecuate

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Biotechnology Research International - Volume 2016 2016, Article ID 3464509, 9 pages -

Research Article

Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt

Department of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University, Cairo 11787, Egypt

Received 2 November 2015; Revised 13 December 2015; Accepted 15 December 2015

Academic Editor: Manuel Canovas

Copyright © 2016 Walaa A. Eraqi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Biosurfactants are produced by bacteria or yeast utilizing different substrates as sugars, glycerol, or oils. They have important applications in the detergent, oil, and pharmaceutical industries. Glycerol is the product of biodiesel industry and the existing glycerol market cannot accommodate the excess amounts generated; consequently, new markets for refined glycerol need to be developed. The aim of present work is to optimize the production of microbial rhamnolipid using waste glycerol. We have developed a process for the production of rhamnolipid biosurfactants using glycerol as the sole carbon source by a local Pseudomonas aeruginosa isolate that was obtained from an extensive screening program. A factorial design was applied with the goal of optimizing the rhamnolipid production. The highest production yield was obtained after 2 days when cells were grown in minimal salt media at pH 6, containing 1% v-v glycerol and 2% w-v sodium nitrate as nitrogen source, at 37°C and at 180 rpm, and reached 2.164 g-L after 54 hours 0.04 g-L h. Analysis of the produced rhamnolipids by TLC, HPLC, and FTIR confirmed the nature of the biosurfactant as monorhamnolipid. Glycerol can serve as a source for the production of rhamnolipid from microbial isolates providing a cheap and reliable substrate.

Author: Walaa A. Eraqi, Aymen S. Yassin, Amal E. Ali, and Magdy A. Amin

Source: https://www.hindawi.com/


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