Ampelopsis Radix Protects Dopaminergic Neurons against 1-Methyl-4-phenylpyridinium-1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-Induced Toxicity in Parkinson’s Disease Models In Vitro and In VivoReport as inadecuate




Ampelopsis Radix Protects Dopaminergic Neurons against 1-Methyl-4-phenylpyridinium-1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-Induced Toxicity in Parkinson’s Disease Models In Vitro and In Vivo - Download this document for free, or read online. Document in PDF available to download.

Evidence-Based Complementary and Alternative MedicineVolume 2013 2013, Article ID 346438, 9 pages

Research Article

Department of Life and Nanopharmaceutical Science, Kyung Hee University, No. 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea

Department of Oriental Pharmaceutical Science, College of Pharmacy and Kyung Hee East-West Pharmaceutical Research Institute, Kyung Hee University, No. 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea

Studies of Translational Acupuncture Research STAR, Acupuncture & Meridian Science Research Center AMSRC, Kyung Hee University, No. 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea

Received 29 May 2013; Revised 2 August 2013; Accepted 20 August 2013

Academic Editor: William C. S. Cho

Copyright © 2013 Hanbyeol Park et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ampelopsis Radix, the root of Ampelopsis japonica Thunb. Makino Vitaceae, is a herbal medicine which has been widely used in East Asia. The present study was done to explore whether the standardized extract of Ampelopsis Radix AJW protects dopaminergic neurons via antioxidant mechanisms in Parkinson’s disease PD models. The effects of AJW on primary mesencephalic cultures stressed with 1-methyl-4-phenylpyridinium were investigated using tyrosine hydroxylase TH immunohistochemistry and reactive oxygen species measurement. The eliminative effects of AJW on the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid radicals were explored using colorimetric methods. The effects of AJW on the mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine MPTP were determined by pole test as well as TH and 8-hydroxydeoxyguanosine immunohistochemistry. AJW protected dopaminergic neurons by inhibiting reactive oxygen species generation in vitro. Moreover, AJW showed potent radical scavenging activities in vitro. In the mouse PD model, AJW protected the dopaminergic neurons in the brain, leading to motor improvements. AJW inhibited the MPTP-evoked accumulation of 8-hydroxydeoxyguanosine in the brain. These data suggest that AJW has neuroprotective effects with antioxidant mechanisms in PD models.





Author: Hanbyeol Park, Jin Sup Shim, Hyo Geun Kim, Hyejung Lee, and Myung Sook Oh

Source: https://www.hindawi.com/



DOWNLOAD PDF




Related documents