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Bioinorganic Chemistry and Applications - Volume 2007 2007, Article ID 36497, 7 pages

Research Article

Department of PG Studies and Research in Industrial Chemistry, School of Chemical Sciences, Kuvempu University, Shankaraghatta, Shimoga, Karnataka 577 451, India

Department of Biotechnology, GM Institute of Technology, Davanagere, Karnataka 577 006, India

Received 8 August 2006; Revised 24 November 2006; Accepted 27 November 2006

Academic Editor: Giovanni Natile

Copyright © 2007 M.
Prabhakara et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


DNA binding and photocleavage characteristics of a series ofmixed ligand complexes of the type Mbpy2qbdpPF6n⋅xH2O where M=CoIII or NiII, bpy=2.2′-bipryidine, qbdp =Quinolino3,2-bbenzodiazepine, n=3 or 2 and x=5 or 2 have beeninvestigated.
The DNA binding property of the complexes with calfthymus DNA has been investigated by using absorption spectra,viscosity measurements, as well as thermal denaturation studies.Intrinsic binding constant Kb has been estimated under similarset of experimental conditions.
Absorption spectral studiesindicate that the CoIII and NiII complexes intercalate betweenthe base pairs of the CT-DNA tightly with intrinsic DNA bindingconstant of 1.3×106 and 3.1×105 M

in Tris-HCl buffer containing50 mM NaCl, respectively.
The proposed DNA binding mode supportsthe large enhancement in the relative viscosity of DNA on bindingto quinolo3,2-bbenzodiazepine.
The oxidative as well as photo-induced cleavage reactions were monitered by gel electrophoresisfor both complexes.
The photocleavage experiments showed thatthe cobaltIII complex can cleave pUC19 DNA effectively in theabsence of external additives as an effective inorganic nuclease.

Autor: M.
Prabhakara, B.
Basavaraju, and H.
Bhojya Naik



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