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Disease Markers - Volume 2015 2015, Article ID 285125, 7 pages -

Research Article

Department of Endocrinology, Fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China

Department of Surgery, Fourth Affiliated Hospital of Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China

Received 27 September 2015; Revised 7 December 2015; Accepted 10 December 2015

Academic Editor: Marco E. M. Peluso

Copyright © 2015 Xi-yu Liu and Jiang-feng Xu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. Latent autoimmune diabetes in adults LADA is the result of gene-environment interactions. Histone acetylation regulates gene expression and maybe interpret how environmental factors modify LADA. Hence, we studied the histone acetylation patterns in CD4

T lymphocytes from LADA patients. Methods. Blood CD4

T lymphocytes from 28 patients with LADA and 28 healthy controls were obtained to detect histone H3 acetylation and H4 acetylation. The gene expression of histone acetyltransferases P300 and CREBBP and histone deacetylases HDAC1, HDAC2, and HDAC7 was measured by real-time polymerase chain reaction RT-PCR. Results. Compared to healthy controls, reduced global H3 acetylation was observed in LADA patients’ CD4

T lymphocytes . Global level of H4 acetylation was not statistically different. Among LADA, CD4

T lymphocytes H3 acetylation was associated with glycosylated hemoglobin HbA1c and GADA titer. Compared to healthy controls, the expression of histone acetyltransferases CREBBP in LADA patients was downregulated, and the expression of histone deacetylases HDAC1 and HDAC7 was upregulated. Conclusion. A concerted downregulation of histone H3 acetylation was found in CD4

T lymphocytes of LADA patients, and this might provide evidence of a novel epigenetic explanation for the pathogenesis of LADA and its complications.





Autor: Xi-yu Liu and Jiang-feng Xu

Fuente: https://www.hindawi.com/



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