Immunohistochemical Expression of Collagens in the Skin of Horses Treated with Leukocyte-Poor Platelet-Rich PlasmaReportar como inadecuado




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BioMed Research International - Volume 2015 2015, Article ID 893485, 12 pages -

Research Article

Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário, Avenida P.H. Rolfs, s-n, 36570-900 Viçosa, MG, Brazil

Clínica Médica Planeta Animal, Rua Araguaia 30, Bairro São Mateus, 36025-240 Juiz de Fora, MG, Brazil

Laboratório de Patologia Experimental, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Rua Sebastião Gonçalves Coelho 400, Bairro Chanadour, 35501-296 Divinópolis, MG, Brazil

Laboratório de Processamento de Tecido, Universidade Federal de São João del-Rei, Campus Centro-Oeste Dona Lindu, Rua Sebastião Gonçalves Coelho 400, Bairro Chanadour, 35501-296 Divinópolis, MG, Brazil

Received 19 November 2014; Revised 5 May 2015; Accepted 12 May 2015

Academic Editor: May Griffith

Copyright © 2015 Maria Verônica de Souza et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study evaluated the immunohistochemical expression of type I COL I and III COL III collagens during the healing process of skin treated with leukocyte-poor platelet-rich plasma LP-PRP. Seven healthy gelding crossbred horses aged 16 to 17 years were used. Two rectangle-shaped wounds were created surgically in the right and left gluteal regions. Twelve hours after wound induction, 0.5 mL of the LP-PRP was administered in each edge of the wounds of one of the gluteal regions. The contralateral region was used as control CG. Three samples were obtained: after wound induction T0, 14 days T1 of healing process, and after complete closure of the skin T2. The normal skin T0 showed strong staining for type III and I collagen in papillary and reticular dermis, respectively. In the scar of the treated group, COL III showed important increase in immunoreaction in T2 compared with T1. The administration of a single dose of LP-PRP 12 h after induction of wound in horses does not influence formation of collagens I and III. However, the intense labeling for COL III suggests that the tissue was still weak during the macroscopic closure of the wound, demonstrating that healing was not completely finished.





Autor: Maria Verônica de Souza, Mariana Brettas Silva, José de Oliveira Pinto, Marianna Barros de Souza Lima, Júlio Crepaldi, Gab

Fuente: https://www.hindawi.com/



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